Article
A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering in Drosophila.
G3 (Bethesda, Md.) - 15 Oct 2014
Zhang Xu, Koolhaas Wouter H, Schnorrer Frank
Abstract excerpt
The development of clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) technologies promises a quantum leap in genome engineering of model organisms. However, CRISPR-mediated gene targeting reports in Drosophila melanogaster are still restricted to a few genes, use variable experimental conditions, and vary in efficiency, questioning the universal applicability of the...
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