Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.

doi:10.1016/j.molcel.2008.06.016

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Article

Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.

Molecular cell - 25 Jul 2008

Maeder Morgan L, Thibodeau-Beganny Stacey, Osiak Anna, Wright David A, Anthony Reshma M, Eichtinger Magdalena, Jiang Tao, Foley Jonathan E, Winfrey Ronnie J, Townsend Jeffrey A, Unger-Wallace Erica, Sander Jeffry D, Müller-Lerch Felix, Fu Fengli, Pearlberg Joseph, Göbel Carl, Dassie Justin P, Pruett-Miller Shondra M, Porteus Matthew H, Sgroi Dennis C, Iafrate A John, Dobbs Drena, McCray Paul B, Cathomen Toni, Voytas Daniel F, Joung J Keith

Abstract excerpt

Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome modifications with high efficiency in cell types including Drosophila, C. elegans, plants, and humans. A bottleneck in the application of ZFN technology has been the generation of highly specific engineered zinc-finger arrays. Here we describe OPEN (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger...

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PMID: 18657511
DOI: 10.1016/j.molcel.2008.06.016
PMCID: PMC2535758

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