Article
A library of TAL effector nucleases spanning the human genome.
Nature biotechnology - 1 Mar 2013
Kim Yongsub, Kweon Jiyeon, Kim Annie, Chon Jae Kyung, Yoo Ji Yeon, Kim Hye Joo, Kim Sojung, Lee Choongil, Jeong Euihwan, Chung Eugene, Kim Doyoung, Lee Mi Seon, Go Eun Mi, Song Hye Jung, Kim Hwangbeom, Cho Namjin, Bang Duhee, Kim Seokjoong, Kim Jin-Soo
Abstract excerpt
Transcription activator-like (TAL) effector nucleases (TALENs) can be readily engineered to bind specific genomic loci, enabling the introduction of precise genetic modifications such as gene knockouts and additions. Here we present a genome-scale collection of TALENs for efficient and scalable gene targeting in human cells. We chose target sites that did not have highly similar sequences elsewhere in the genome...
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