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Article

Targeted mutagenesis of duplicated genes in soybean with zinc-finger nucleases.

Plant physiology - 1 Jun 2011

Curtin Shaun J, Zhang Feng, Sander Jeffry D, Haun William J, Starker Colby, Baltes Nicholas J, Reyon Deepak, Dahlborg Elizabeth J, Goodwin Mathew J, Coffman Andrew P, Dobbs Drena, Joung J Keith, Voytas Daniel F, Stupar Robert M

Abstract excerpt

We performed targeted mutagenesis of a transgene and nine endogenous soybean (Glycine max) genes using zinc-finger nucleases (ZFNs). A suite of ZFNs were engineered by the recently described context-dependent assembly platform--a rapid, open-source method for generating zinc-finger arrays. Specific ZFNs targeting dicer-like (DCL) genes and other genes involved in RNA silencing were cloned into a vector under an...

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PMID: 21464476
DOI: 10.1104/pp.111.172981
PMCID: PMC3177250

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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