Article
Effects of mcr restriction of methylated CpG islands of the L1 transposons during packaging and plating stages of mammalian genomic library construction.
Gene - 1 Feb 1991
Doherty J P, Graham M W, Linsenmeyer M E, Crowther P J, Williamson M, Woodcock D M
Abstract excerpt
The use of optimally methylation-tolerant mcrA- mcrB- strains has been shown to produce an over tenfold increase in the plating efficiencies of mammalian genomic libraries, compared to a superior conventional phage host strain LE392 which is mcrB+. However, there is an even more significant effect of mcr restriction. Amongst the recombinants recovered with an mcrB+ host, we have found that there is an additional...
Topics
- Animals
- Bacteriophages
- Chromosome Deletion
- Cloning, Molecular
- DNA Transposable Elements
- Deoxyribonucleases
- Dinucleoside Phosphates
- Escherichia coli
- Genes, Bacterial
- Genetic Vectors