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Article

Modified-cytosine restriction-system-induced recombinant cloning artefacts in Escherichia coli.

Gene - 14 Feb 1993

Williamson M R, Doherty J P, Woodcock D M

Abstract excerpt

We have tested whether, and to what extent, recombinant clones from DNA segments with 5-methylation of cytosines recovered in methylation-restrictive (mcr+) hosts contain mutations. We constructed a model system in which the tetracycline-resistance-encoding gene (tet) from pBR322 was cloned into the plasmid pGEM3Zf+. The central region of tet was removed from the construct, methylated in vitro and then religated...

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Identifiers

PMID: 8382656
DOI: 10.1016/0378-1119(93)90759-v

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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