Modified-cytosine restriction-system-induced recombinant cloning artefacts in Escherichia coli.

doi:10.1016/0378-1119(93)90759-v

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Article

Modified-cytosine restriction-system-induced recombinant cloning artefacts in Escherichia coli.

Gene - 14 Feb 1993

Williamson M R, Doherty J P, Woodcock D M

Abstract excerpt

We have tested whether, and to what extent, recombinant clones from DNA segments with 5-methylation of cytosines recovered in methylation-restrictive (mcr+) hosts contain mutations. We constructed a model system in which the tetracycline-resistance-encoding gene (tet) from pBR322 was cloned into...

Topics

5-Methylcytosine
Base Sequence
Cloning, Molecular
Cytosine
DNA Restriction Enzymes
DNA, Bacterial
Dinucleoside Phosphates
Escherichia coli
Genes, Bacterial
Genotype
Mutation
Plasmids
Recombination, Genetic
Tetracycline Resistance

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