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Article

Transposon-mediated linker insertion scanning mutagenesis of the Escherichia coli McrA endonuclease.

Journal of bacteriology - 1 Sept 2004

Anton Brian P, Raleigh Elisabeth A

Abstract excerpt

McrA is one of three functions that restrict modified foreign DNA in Escherichia coli K-12, affecting both methylated and hydroxymethylated substrates. We present here the first systematic analysis of the functional organization of McrA by using the GPS-LS insertion scanning system. We collected in-frame insertions of five amino acids at 46 independent locations and C-terminal truncations at 20 independent...

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Identifiers

PMID: 15317774
DOI: 10.1128/JB.186.17.5699-5707.2004
PMCID: PMC516834

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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