Escherichia coli host strains SURE and SRB fail to preserve a palindrome cloned in lambda phage: improved alternate host strains.

doi:10.1016/0378-1119(93)90758-u

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Article

Escherichia coli host strains SURE and SRB fail to preserve a palindrome cloned in lambda phage: improved alternate host strains.

Gene - 14 Feb 1993

Doherty J P, Lindeman R, Trent R J, Graham M W, Woodcock D M

Abstract excerpt

We have attempted to produce Escherichia coli strains with the optimal combination of host mutations required for the construction of genomic libraries in lambda and cosmid vectors. For lambda vectors, we defined this as a strain that combined high efficiency of phage plating with optimal toleran...

Topics

Bacteriophage lambda
Base Sequence
Cloning, Molecular
Cosmids
Escherichia coli
Genes, Bacterial
Genetic Vectors
Genomic Library
Genotype
Humans
Mutagenesis
Recombination, Genetic

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