Article
High frequency mutagenesis by a DNA methyltransferase.
Cell - 24 Dec 1992
Shen J C, Rideout W M, Jones P A
Abstract excerpt
HpaII methylase (M. HpaII), an example of a DNA (cytosine-5)-methyltransferase, was found to induce directly a high frequency of C-->U transition mutations in double-stranded DNA. A mutant pSV2-neo plasmid, constructed with an inactivating T-->C transition mutation creating a CCGG site, was incubated with M. HpaII in the absence of S-adenosylmethionine (SAM). This caused an approximately 10(4)-fold increase in...
Topics
- Base Sequence
- Cytosine
- DNA
- DNA-Cytosine Methylases
- Deamination
- Methylation
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- S-Adenosylhomocysteine