Article
A novel activity in Escherichia coli K-12 that directs restriction of DNA modified at CG dinucleotides.
Journal of bacteriology - 1 Aug 1991
Kelleher J E, Raleigh E A
Abstract excerpt
The restriction systems McrA and McrB of Escherichia coli K-12 are known to attack DNA containing modified cytosine. In strains lacking both activities, however, we observed that DNA methylated at CG dinucleotides (as is mammalian DNA) was still significantly restricted. We show that this substantial barrier to the acceptance of 5-methylcytosine-containing DNA is attributable to a hitherto unknown activity of the...
Topics
- 5-Methylcytosine
- Bacteriophage lambda
- Cloning, Molecular
- Cytosine
- DNA
- DNA Restriction-Modification Enzymes
- Dinucleoside Phosphates
- Escherichia coli
- Mutation