Article
Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990
Grant S G, Jessee J, Bloom F R, Hanahan D
Abstract excerpt
Plasmids comprising transgene insertions in four lines of transgenic mice have been retrieved by plasmid rescue into a set of Escherichia coli strains with mutations in different members of the methylation-dependent restriction system (MDRS). Statistical analysis of plasmid rescue frequencies has revealed that the MDRS loci detect differential modifications of the transgene insertions among mouse lines that show...
Topics
- Animals
- Chromosome Mapping
- DNA
- DNA Transposable Elements
- Escherichia coli
- Genotype
- Methylation
- Mice
- Mice, Transgenic
- Mutation
- Plasmids