Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12.

doi:10.1128/jb.173.1.150-155.1991

Back to search

Article

Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12.

Journal of bacteriology - 1 Jan 1991

Piekarowicz A, Yuan R, Stein D C

Abstract excerpt

We isolated temperature-sensitive mcrA and mcrBC mutants of Escherichia coli. At 42 degrees C, they were unable to restrict the T-even bacteriophages T6gt and T4gt or plasmids encoding cloned DNA methylase genes whose specificities confer sensitivity to the McrA and McrBC nucleases. Complementati...

Topics

Chromosomes, Bacterial
DNA Restriction Enzymes
Escherichia coli
Genetic Complementation Test
Genotype
Mutagenesis
Mutation
Nitrosoguanidines
Phenotype
Plasmids
Restriction Mapping
Temperature

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.