Article
Peptide induction of surface expression of class I MHC.
Current protocols in immunology - 1 Nov 2003
Hansen Ted, Myers Nancy
Abstract excerpt
This unit describes a method for comparing the relative binding of different peptides to the same MHC class I (MHC-I) molecule using live cells. Live cells expressing suboptimally loaded MHC-I proteins are incubated with medium containing diluted amounts of synthetic peptides to be tested for binding to class I. After overnight incubation with peptide, surface class I expression is monitored by flow cytometry...
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