A method to quantify binding of unlabeled peptides to class I MHC molecules and detect their allele specificity.

doi:10.1016/0022-1759(93)90210-x

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Article

A method to quantify binding of unlabeled peptides to class I MHC molecules and detect their allele specificity.

Journal of immunological methods - 3 Feb 1993

Elvin J, Potter C, Elliott T, Cerundolo V, Townsend A

Abstract excerpt

A general method has been developed for measuring the stabilization of class I MHC molecules in extracts of the mutant cell lines .174/T2 and RMA-S. 35S-Met-labeled class I molecules which have been stabilized by peptides in vitro are immunoprecipitated with conformation dependent monoclonal antibodies and electrophoresed on polyacrylamide gels. The heavy and light chains are excised from the dried gel and...

Topics

Alleles
Amino Acid Sequence
Animals
Antibody Affinity
Antibody Specificity
Antigen-Antibody Reactions
Cell Line
Electrophoresis, Polyacrylamide Gel
Epitopes
Gene Products, gag
HIV Antigens

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Histocompatibility Antigens Class I

Humans

Isoelectric Focusing

Ligands

Mice

Molecular Sequence Data

Nucleocapsid Proteins

Source

Open in PubMed

Identifiers

PMID: 7679131
DOI: 10.1016/0022-1759(93)90210-x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7679131-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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