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Article

A high throughput MHC II binding assay for quantitative analysis of peptide epitopes.

Journal of visualized experiments : JoVE - 25 Mar 2014

Salvat Regina, Moise Leonard, Bailey-Kellogg Chris, Griswold Karl E

Abstract excerpt

Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design(1,2). Here, a peptide-MHC II binding assay is scaled to 384-well format. The scaled down protocol reduces reagent costs by 75% and is higher throughput than previously described 96-well protocols(1,3-5). Specifically, the experimental design permits...

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Identifiers

PMID: 24686319
DOI: 10.3791/51308
PMCID: PMC4027027

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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