Article
A novel genetic selection system for improved enantioselectivity of Bacillus subtilis lipase A.
Chembiochem : a European journal of chemical biology - 5 May 2008
Boersma Ykelien L, Dröge Melloney J, van der Sloot Almer M, Pijning Tjaard, Cool Robbert H, Dijkstra Bauke W, Quax Wim J
Abstract excerpt
In directed evolution experiments, success often depends on the efficacy of screening or selection methods. Genetic selections have proven to be extremely valuable for evolving enzymes with improved catalytic activity, improved stability, or with altered substrate specificity. In contrast, enantioselectivity is a difficult parameter to select for. In this study, we present a successful strategy that not only...
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