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Article

Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.

Proceedings of the National Academy of Sciences of the United States of America - 3 Jan 2012

Sandström Anders G, Wikmark Ylva, Engström Karin, Nyhlén Jonas, Bäckvall Jan-E

Abstract excerpt

A highly combinatorial structure-based protein engineering method for obtaining enantioselectivity is reported that results in a thorough modification of the substrate binding pocket of Candida antarctica lipase A (CALA). Nine amino acid residues surrounding the entire pocket were simultaneously mutated, contributing to a reshaping of the substrate pocket to give increased enantioselectivity and activity for a...

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Identifiers

PMID: 22178758
DOI: 10.1073/pnas.1111537108
PMCID: PMC3252943

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