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Article

Manipulating the expression rate and enantioselectivity of an epoxide hydrolase by using directed evolution.

Chembiochem : a European journal of chemical biology - 4 Jul 2011

Reetz Manfred T, Zheng Huabao

Abstract excerpt

We describe here a strategy to improve the expression efficiency and enantioselectivity of Aspergillus niger epoxide hydrolase (ANEH) by directed evolution. Based on a blue-colony screening system using the LacZα (β-galactosidase α peptide) complementation solubility reporter, several ANEH variants out of 15 000 transformants from a random-mutagenesis library were identified that show improved recombinant...

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Identifiers

PMID: 21567703
DOI: 10.1002/cbic.201100078

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