Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations.

doi:10.1007/s00253-016-8035-1

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Article

Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations.

Applied microbiology and biotechnology - 1 Apr 2017

Zhao Jing, Frauenkron-Machedjou Victorine Josiane, Kardashliev Tsvetan, Ruff Anna Joëlle, Zhu Leilei, Bocola Marco, Schwaneberg Ulrich

Abstract excerpt

The quality of amino acid substitution patterns in random mutagenesis libraries is decisive for the success in directed evolution campaigns. In this manuscript, we provide a detailed analysis of the amino acid substitutions by analyzing 3000 mutations of three random mutagenesis libraries (1000 mutations each; epPCR with a low-mutation and a high-mutation frequency and SeSaM-Tv P/P) employing lipase A from...

Topics

Amino Acid Substitution
Bacillus subtilis
DNA Mutational Analysis
Directed Molecular Evolution
Gene Library
Lipase
Mutagenesis
Mutation
Polymerase Chain Reaction
Protein Engineering

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Open in PubMed

Identifiers

PMID: 28050632
DOI: 10.1007/s00253-016-8035-1

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