Article
Improving the expression yield of Candida antarctica lipase B in Escherichia coli by mutagenesis.
Biotechnology letters - 1 Apr 2008
Jung Suhyun, Park Seongsoon
Abstract excerpt
Increasing the expression yield of active Candida antarctica lipase B (CAL-B) in Escherichia coli was achieved by using a codon-optimized synthetic gene and by mutagenesis to introduce hydrophilic residues on the surface of CAL-B. Five residues (four leucines and one isoleucine) on the surface of CAL-B were selected and changed with aspartate after codon optimization. While the codon-optimized synthetic gene of...
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