Article
Loop grafting of Bacillus subtilis lipase A: inversion of enantioselectivity.
Chemistry & biology - 25 Aug 2008
Boersma Ykelien L, Pijning Tjaard, Bosma Margriet S, van der Sloot Almer M, Godinho Luís F, Dröge Melloney J, Winter Remko T, van Pouderoyen Gertie, Dijkstra Bauke W, Quax Wim J
Abstract excerpt
Lipases are successfully applied in enantioselective biocatalysis. Most lipases contain a lid domain controlling access to the active site, but Bacillus subtilis Lipase A (LipA) is a notable exception: its active site is solvent exposed. To improve the enantioselectivity of LipA in the kinetic resolution of 1,2-O-isopropylidene-sn-glycerol (IPG) esters, we replaced a loop near the active-site entrance by longer...
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