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Expanding the substrate scope of enzymes: combining mutations obtained by CASTing.

Chemistry (Weinheim an der Bergstrasse, Germany) - 7 Aug 2006

Reetz Manfred T, Carballeira José Daniel, Peyralans Jérôme, Höbenreich Horst, Maichele Andrea, Vogel Andreas

Abstract excerpt

In a previous paper, the combinatorial active-site saturation test (CAST) was introduced as an effective strategy for the directed evolution of enzymes toward broader substrate acceptance. CASTing comprises the systematic design and screening of focused libraries around the complete binding pocket, but it is only the first step of an evolutionary process because only the initial libraries of mutants are...

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PMID: 16789057
DOI: 10.1002/chem.200600459

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CAST

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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