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Article

Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection.

Chembiochem : a European journal of chemical biology - 1 Jan 2006

Dröge Melloney J, Boersma Ykelien L, van Pouderoyen Gertie, Vrenken Titia E, Rüggeberg Carsten J, Reetz Manfred T, Dijkstra Bauke W, Quax Wim J

Abstract excerpt

Phage display can be used as a protein-engineering tool for the selection of proteins with desirable binding properties from a library of mutants. Here we describe the application of this method for the directed evolution of Bacillus subtilis lipase A, an enzyme that has important properties for the preparation of the pharmaceutically relevant chiral compound 1,2-O-isopropylidene-sn-glycerol (IPG). PCR...

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PMID: 16342303
DOI: 10.1002/cbic.200500308

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