Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line.

Abstract excerpt

Single microRNA (miRNA) can be inhibited using antagomiR which efficiently knocks down a specific miRNA. However, the effect is transient and often results in subtle phenotype. Here we report a guideline on designing miRNA sponges inhibiting a miRNA family. As a model system, we targeted miR-30 family, known as tumor suppressor miRNAs in multiple tumors. To achieve an efficient knockdown, we generated perfect and...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Cell Line
• Share to MicroRNAs
• Share to Models, Biological