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Article

Flow cytometry-based functional selection of RNA interference triggers for efficient epi-allelic analysis of therapeutic targets.

BMC biotechnology - 21 Jun 2014

Micklem David R, Blø Magnus, Bergström Petra, Hodneland Erlend, Tiron Crina, Høiby Torill, Gjerdrum Christine, Hammarsten Ola, Lorens James B

Abstract excerpt

BACKGROUND: The dose-response relationship is a fundamental pharmacological parameter necessary to determine therapeutic thresholds. Epi-allelic hypomorphic analysis using RNA interference (RNAi) can similarly correlate target gene dosage with cellular phenotypes. This however requires a set of RNAi triggers empirically determined to attenuate target gene expression to different levels. RESULTS: In order to...

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Identifiers

PMID: 24952598
DOI: 10.1186/1472-6750-14-57
PMCID: PMC4074332

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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