Article
Single-strand conformation polymorphisms can be used to detect T cell receptor gene rearrangements: an application to the in vivo hprt mutation assay.
Mutagenesis - 1 Sept 1991
Caggana M, Benjamin M B, Little J B, Liber H L, Kelsey K T
Abstract excerpt
Epidemiologic application of the human in vivo hypoxanthine-guanine phosphoribosyltransferase (hprt) mutation assay requires screening of mutant colonies to differentiate independent from clonal origin. Previously, sibship was defined by Southern blot analysis of T cell receptor gene rearrangements. We report here a more expedient method to determine these rearrangements utilizing the polymerase chain reaction...
Topics
- Base Sequence
- Blotting, Southern
- DNA, Single-Stranded
- Gene Rearrangement, T-Lymphocyte
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Polymerase Chain Reaction