Article
Alterations of the hprt gene in human in vivo-derived 6-thioguanine-resistant T lymphocytes.
Nature - 1 Jan 2000
Albertini R J, O'Neill J P, Nicklas J A, Heintz N H, Kelleher P C
Abstract excerpt
Investigations into the extent and significance of somatic gene mutations occurring in vivo in humans have been hampered by the lack of a means of unambiguously defining the mutational origin of in vivo-derived variant cells. Several years ago we proposed that 6-thioguanine-resistant T lymphocytes, present at low frequencies in human peripheral blood, might be useful markers of in vivo somatic mutation. We and...
Topics
- Clone Cells
- DNA
- DNA Restriction Enzymes
- Drug Resistance
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Male
- Mutation
- Phenotype
- T-Lymphocytes
- Thioguanine