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Article

Design of an optimal allele-specific oligonucleotide probe for the efficient discrimination of a thermodynamically stable (G x T) mismatch.

Analytica chimica acta - 5 Nov 2007

Lucarelli Fausto, Marrazza Giovanna, Mascini Marco

Abstract excerpt

A general approach that allows reliable detection of a single base-pair mismatch is presented. Specifically, how important a careful design of the allele-specific oligonucleotide (ASO) probe is, was demonstrated for a G x T mispair highly stable from the thermodynamic point of view. This study involved comparison of six ASO probes of variable length (25 to 9-mer), with a similar GC composition and designed to...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 17950061
DOI: 10.1016/j.aca.2007.09.047

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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