Loop-recognition dual-hairpin competitive probes enabling a dual-ratiometric electrochemical platform for specific SNP discrimination and logic gate construction.

Abstract excerpt

Accurate discrimination of single nucleotide polymorphisms (SNPs) remains a fundamental challenge in nucleic acid biosensing due to the minimal thermodynamic differences between perfectly matched and single-base mismatched sequences. This intrinsic limitation often compromises recognition fidelity at the initial hybridization stage, regardless of subsequent signal amplification strategies. Herein, we propose a...

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