G/T mismatch discrimination by positioning the mismatch site close to the catalytic core of a 10-23 DNAzyme sensor.

Abstract excerpt

Single nucleotide variation (SNV) detection is crucial for the diagnosis of several diseases. A 10-23 binary DNAzyme-based sensor was able to differentiate the challenging G → A substitution with an initial differentiation factor of >83%, which increased to >98% after further optimization, when the SNV site was positioned close to the DNAzyme catalytic core, introducing an SNV discrimination principle not solely...

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