Article
Rationally Designed Dual Base Pair Mismatch Enables Toehold-Mediated Strand Displacement to Efficiently Recognize Single-Nucleotide Polymorphism without Enzymes.
Analytical chemistry - 9 Jan 2024
Zhang Yunshan, Wang Lanyue, Ye Jing, Chen Jian, Xu Shijie, Bu Sisi, Deng Minxin, Bian Lina, Zhao Xiaoyu, Zhang Chunlong, Weng Lin, Zhang Diming
Abstract excerpt
The efficiency of the enzyme-free toehold-mediated strand displacement (TMSD) technique is often insufficient to detect single-nucleotide polymorphism (SNP) that possesses only single base pair mismatch discrimination. Here, we report a novel dual base pair mismatch strategy enabling TMSD biosensing for SNP detection under enzyme-free conditions when coupled with catalytic hairpin assembly (CHA) and fluorescence...
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