Article
Secondary structures as predictors of mutation potential in the lacZ gene of Escherichia coli.
Microbiology (Reading, England) - 1 Jul 2007
Burkala Evan, Reimers Jacqueline M, Schmidt Karen H, Davis Nick, Wei Ping, Wright Barbara E
Abstract excerpt
Four independent nonsense mutations were engineered into the Escherichia coli chromosomal lacZ gene, and reversion rates back to LacZ(+) phenotypes were determined. The mutation potential of bases within putative DNA secondary structures formed during transcription was predicted by a sliding-window analysis that simulates successive folding of the ssDNA creating these structures. The relative base mutabilities...
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