Article
The effect of promoter strength, supercoiling and secondary structure on mutation rates in Escherichia coli.
Molecular microbiology - 1 Jun 2006
Schmidt Karen H, Reimers Jacqueline M, Wright Barbara E
Abstract excerpt
Four mutations resulting in opal stop codons were individually engineered into a plasmid-borne chloramphenicol-resistance (cat) gene driven by the lac promoter. These four mutations were located at different sites in secondary structures. The mutations were analysed with the computer program mfg, which predicted their relative reversion frequencies. Reversion frequencies determined experimentally correlated with...
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