Article
Bisulfite-induced cytosine deamination rates in E. coli SSB:DNA complexes.
Mutation research - 1 Jul 2001
Lough J, Jackson M, Morris R, Moyer R
Abstract excerpt
E. coli single-stranded binding protein (SSB) has been examined for its ability to modulate bisulfite-induced cytosine deamination rates in single-stranded DNA (ssDNA). We used a lacZ alpha-complementation reversion assay to detect C-->U rates at a single codon in M13mp2 DNA, whether in free ssDNA or in an SSB:ssDNA complex. When incubated at 37 degrees C, the average bisulfite-induced reversion rate constant was...
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