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Article

A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations.

Genetics - 1 Jun 1990

Cupples C G, Cabrera M, Cruz C, Miller J H

Abstract excerpt

We have used site-directed mutagenesis to alter bases in lacZ near the region encoding essential residues in the active site of beta-galactosidase. The altered sequences generate runs of six or seven identical base pairs which create a frameshift, resulting in a Lac- phenotype. Reversion to Lac+ in each strain can occur only by a specific frameshift at these sequences. Monotonous runs of A's (or of T's on the...

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Identifiers

PMID: 2199309
DOI: 10.1093/genetics/125.2.275
PMCID: PMC1204017

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Source receipt: pubmed, retrieved 7 Sept 2026.

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