A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations.

doi:10.1093/genetics/125.2.275

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Article

A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations.

Genetics - 1 Jun 1990

Cupples C G, Cabrera M, Cruz C, Miller J H

Abstract excerpt

We have used site-directed mutagenesis to alter bases in lacZ near the region encoding essential residues in the active site of beta-galactosidase. The altered sequences generate runs of six or seven identical base pairs which create a frameshift, resulting in a Lac- phenotype. Reversion to Lac+...

Topics

Base Sequence
Escherichia coli
Lac Operon
Molecular Sequence Data
Mutagens
Mutation
Phenotype

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