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Article

Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting.

The Journal of molecular diagnostics : JMD - 1 Jul 2007

Seipp Michael T, Durtschi Jacob D, Liew Michael A, Williams Jamie, Damjanovich Kristy, Pont-Kingdon Genevieve, Lyon Elaine, Voelkerding Karl V, Wittwer Carl T

Abstract excerpt

Amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, or allele-specific polymerase chain reaction. However, correct differentiation of homozygous mutant and wild-type samples by melting temperature (Tm) requires high-resolution melting and closely controlled reaction conditions. When three different DNA extraction methods were used to isolate DNA from whole...

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Identifiers

PMID: 17591926
DOI: 10.2353/jmoldx.2007.060136
PMCID: PMC1899416

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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