Article
Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping.
Clinical chemistry - 1 Aug 2007
Herrmann Mark G, Durtschi Jacob D, Wittwer Carl T, Voelkerding Karl V
Abstract excerpt
BACKGROUND: Additional instruments have become available since instruments for DNA melting analysis of PCR products for genotyping and mutation scanning were compared. We assessed the performance of these new instruments for genotyping and scanning for mutations. METHODS: A 110-bp fragment of the beta-globin gene including the sickle cell anemia locus (HBB c. 20A>T) was amplified by PCR in the presence of LCGreen...
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