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Article

High-Speed Melting Analysis: The Effect of Melting Rate on Small Amplicon Microfluidic Genotyping.

Clinical chemistry - 1 Oct 2017

Pryor Robert J, Myrick Joseph T, Palais Robert A, Sundberg Scott O, Paek Jeanette Y, Wittwer Carl T, Knight Ivor T

Abstract excerpt

BACKGROUND: High-resolution DNA melting analysis of small amplicons is a simple and inexpensive technique for genotyping. Microfluidics allows precise and rapid control of temperature during melting. METHODS: Using a microfluidic platform for serial PCR and melting analysis, 4 targets containing single nucleotide variants were amplified and then melted at different rates over a 250-fold range from 0.13 to 32...

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PMID: 28818830
DOI: 10.1373/clinchem.2017.276147

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