Article
Selectivity and proofreading both contribute significantly to the fidelity of RNA polymerase III transcription.
Proceedings of the National Academy of Sciences of the United States of America - 19 Jun 2007
Alic Nazif, Ayoub Nayla, Landrieux Emilie, Favry Emmanuel, Baudouin-Cornu Peggy, Riva Michel, Carles Christophe
Abstract excerpt
We examine here the mechanisms ensuring the fidelity of RNA synthesis by RNA polymerase III (Pol III). Misincorporation could only be observed by using variants of Pol III deficient in the intrinsic RNA cleavage activity. Determination of relative rates of the reactions producing correct and erroneous transcripts at a specific position on a tRNA gene, combined with computational methods, demonstrated that Pol III...
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