Factor-specific effects of mutations in the active site of RNA polymerase on RNA cleavage.

Abstract excerpt

Bacterial RNA polymerase (RNAP) relies on the same active site for RNA synthesis and co-transcriptional RNA proofreading. The intrinsic RNA proofreading activity of RNAP can be greatly stimulated by Gre factors, which bind within the secondary channel and directly participate in the RNA cleavage reaction in the active site of RNAP. Here, we characterize mutations in Escherichia coli RNAP that differentially...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Binding Sites
• Share to DNA-Directed RNA Polymerases