Article
The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.
The Journal of biological chemistry - 15 Aug 1990
Bebenek K, Joyce C M, Fitzgerald M P, Kunkel T A
Abstract excerpt
The fidelity of DNA synthesis by an exonuclease-proficient DNA polymerase results from the selectivity of the polymerization reaction and from exonucleolytic proofreading. We have examined the contribution of these two steps to the fidelity of DNA synthesis catalyzed by the large Klenow fragment of Escherichia coli DNA polymerase I, using enzymes engineered by site-directed mutagenesis to inactivate the...
Topics
- Base Sequence
- Codon
- DNA Polymerase I
- DNA, Bacterial
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Templates, Genetic