Article
Purification and properties of wild-type and exonuclease-deficient DNA polymerase II from Escherichia coli.
The Journal of biological chemistry - 23 Jun 1995
Cai H, Yu H, McEntee K, Kunkel T A, Goodman M F
Abstract excerpt
Wild-type DNA polymerase II (pol II) and an exonuclease-deficient pol II mutant (D155A/E157A) have been overexpressed and purified in high yield from Escherichia coli. Wild-type pol II exhibits a high proofreading 3'-exonuclease to polymerase ratio, similar in magnitude to that observed for bacte...
Topics
- Base Sequence
- Chromatography, Ion Exchange
- DNA Polymerase II
- DNA Primers
- DNA-Directed DNA Polymerase
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Exonucleases
- Frameshift Mutation
- Genes, Bacterial
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Point Mutation
- Sequence Deletion
- Templates, Genetic