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Article

Purification and properties of wild-type and exonuclease-deficient DNA polymerase II from Escherichia coli.

The Journal of biological chemistry - 23 Jun 1995

Cai H, Yu H, McEntee K, Kunkel T A, Goodman M F

Abstract excerpt

Wild-type DNA polymerase II (pol II) and an exonuclease-deficient pol II mutant (D155A/E157A) have been overexpressed and purified in high yield from Escherichia coli. Wild-type pol II exhibits a high proofreading 3'-exonuclease to polymerase ratio, similar in magnitude to that observed for bacteriophage T4 DNA polymerase. While copying a 250-nucleotide region of the lacZ alpha gene, the fidelity of wild-type pol...

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PMID: 7797520
DOI: 10.1074/jbc.270.25.15327

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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