Article
Efficiency of correct nucleotide insertion governs DNA polymerase fidelity.
The Journal of biological chemistry - 6 Dec 2002
Beard William A, Shock David D, Vande Berg Brian J, Wilson Samuel H
Abstract excerpt
DNA polymerase fidelity or specificity expresses the ability of a polymerase to select a correct nucleoside triphosphate (dNTP) from a pool of structurally similar molecules. Fidelity is quantified from the ratio of specificity constants (catalytic efficiencies) for alternate substrates (i.e. correct and incorrect dNTPs). An analysis of the efficiency of dNTP (correct and incorrect) insertion for a low fidelity...
Topics
- Binding Sites
- Catalysis
- DNA
- DNA Damage
- DNA Polymerase beta
- DNA Repair
- Humans
- Kinetics
- Models, Molecular
- Mutation
- Oligonucleotides
- Protein Binding
- Protein Structure, Tertiary