Article
8-Oxo-2'-deoxyguanosine Replication in Mutational Hot Spot Sequences of the p53 Gene in Human Cells Is Less Mutagenic than That of the Corresponding Formamidopyrimidine.
Chemical research in toxicology - 15 May 2023
Stanio Stephen, Bacurio Jan Henric T, Yang Haozhe, Greenberg Marc M, Basu Ashis K
Abstract excerpt
7,8-Dihydro-8-oxo-2'-deoxyguanosine (8-OxodGuo) is a ubiquitous DNA damage formed by oxidation of 2'-deoxyguanosine. In this study, plasmid DNA containing 8-OxodGuo located in three mutational hot spots of human cancers, codons 248, 249, and 273 of the Tp53 tumor suppressor gene, was replicated in HEK 293T cells. 8-OxodGuo was only a weak block of replication, and the bypass was largely error-free. The mutations...
Topics
- Humans
- 8-Hydroxy-2'-Deoxyguanosine
- Mutagens
- Genes, p53
- Mutation
- Mutagenesis
- DNA Replication
- DNA Damage
- Deoxyguanosine