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Article

Several concerns about the primer design in the universal molecular beacon real-time PCR assay and its application in HBV DNA detection.

Analytical and bioanalytical chemistry - 1 Jun 2007

Li Xiaomin, Huang Yong, Song Chen, Zhao Meiping, Li Yuanzong

Abstract excerpt

A universal hepatitis B virus (HBV) DNA detection kit is appealing for the worldwide diagnosis and monitoring of the treatment of different mutant types of hepatitis B virus. A sensitive and reproducible real-time PCR assay based on the universal molecular beacon (U-MB) technique was developed for the detection of HBV DNA in serum. The U-MB probe used in the assay has no interaction with the HBV DNA sequence. The...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17468857
DOI: 10.1007/s00216-007-1281-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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