Article
Designing primers from multiple sequences using Matchup program to improve detection of hepatitis B virus by polymerase chain reaction.
Journal of microbiology (Seoul, Korea) - 1 Feb 2010
Jang So Young, Kim Mi Suk, Park Min Seok, Lee Keon Myung, Chung Hwan Won, Chun Jongsik, Lee Chan Hee
Abstract excerpt
Traditionally primers for PCR detection of viruses have been selected from genomic sequence of single or representative viral strain. However, high mutation rate of viral genomes often results in failure in detecting viruses in clinical and environmental samples. Thus, it seems necessary to consider primers designed from multiple viral sequences in order to improve detection of viral variants. Matchup is a...
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