Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Hepatitis C Virus RNA Real-Time Quantitative RT-PCR Method Based on a New Primer Design Strategy.

The Journal of molecular diagnostics : JMD - 1 Jan 2016

Chen Lida, Li Wenli, Zhang Kuo, Zhang Rui, Lu Tian, Hao Mingju, Jia Tingting, Sun Yu, Lin Guigao, Wang Lunan, Li Jinming

Abstract excerpt

Viral nucleic acids are unstable when improperly collected, handled, and stored, resulting in decreased sensitivity of currently available commercial quantitative nucleic acid testing kits. Using known unstable hepatitis C virus RNA, we developed a quantitative RT-PCR method based on a new primer design strategy to reduce the impact of nucleic acid instability on nucleic acid testing. The performance of the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Hepacivirus
Share to Hepatitis C

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Limit of Detection

Share to Nucleic Acid Amplification Techniques

Share to Real-Time Polymerase Chain Reaction

Share to RNA Stability

Share to Viral Load

Source

Identifiers

PMID: 26612712
DOI: 10.1016/j.jmoldx.2015.07.009

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Hepatitis C Virus RNA Real-Time Quantitative RT-PCR Method Based on a New Primer Design Strategy.PMID 26612712Read ↗

Overestimation and underestimation of hepatitis C virus RNA levels in a widely used real-time polymerase chain reaction-based method.Semantic proximity Read ↗

Development of novel triplex single-step real-time PCR assay for detection of Hepatitis Virus B and C simultaneously.Semantic proximity Read ↗

Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I.Semantic proximity Read ↗

Design of an antisense reverse-transcriptase-polymerase chain reaction primer efficient for all hepatitis C virus genotypes: comparison of its performance vs a commercial primer.Semantic proximity Read ↗

Development of a low-cost real-time reverse transcriptase-polymerase chain reaction technique for the detection and quantification of hepatitis C viral load.Semantic proximity Read ↗

Improved TaqMan real-time assays for detecting hepatitis A virus.Semantic proximity Read ↗

Real-time detection system for quantification of hepatitis C virus genome.Semantic proximity Read ↗

Multiplex real-time reverse transcription-PCR assay for determination of hepatitis C virus genotypes.Semantic proximity Read ↗

Differences between two real-time PCR-based hepatitis C virus (HCV) assays (RealTime HCV and Cobas AmpliPrep/Cobas TaqMan) and one signal amplification assay (Versant HCV RNA 3.0) for RNA detection and quantification.Semantic proximity Read ↗

COBAS ® AmpliPrep/COBAS ® Taqman ® HCV Quantitative Test, version 2.0: an in vitro test for hepatitis C virus RNA quantification.Semantic proximity Read ↗

Second-generation Cobas AmpliPrep/Cobas TaqMan HCV quantitative test for viral load monitoring: a novel dual-probe assay design.Semantic proximity Read ↗

A complete molecular biology assay for hepatitis C virus detection, quantification and genotyping.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.