Article
Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman-ARMS Followed a Strict Control System.
Journal of clinical laboratory analysis - 1 Jul 2016
Lu Renfei, Shao Jianguo, Zhou Min, Zhang Hongping, Wu Yueping
Abstract excerpt
BACKGROUND: Hepatitis B virus (HBV) G1896A mutation was associated with HBeAg seronegativity and hepatitis B related acute-on-chronic liver failure. In this study, we developed Taqman amplification refractory mutation system (Taqman-ARMS) and established a strict control system to detect HBV G1896A mutant. METHODS: HBV viral DNA was isolated from 60 patient serum samples, and full-length HBV genome was cloned....
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