Article
Establishment of a novel two-probe real-time PCR for simultaneously quantification of hepatitis B virus DNA and distinguishing genotype B from non-B genotypes.
Clinica chimica acta; international journal of clinical chemistry - 1 Nov 2014
Wang Wei, Liang Hongpin, Zeng Yongbin, Lin Jinpiao, Liu Can, Jiang Ling, Yang Bin, Ou Qishui
Abstract excerpt
OBJECTIVE: Establishment of a simple, rapid and economical method for quantification and genotyping of hepatitis B virus (HBV) is of great importance for clinical diagnosis and treatment of chronic hepatitis B patients. We hereby aim to develop a novel two-probe real-time PCR for simultaneous quantification of HBV viral concentration and distinguishing genotype B from non-B genotypes. METHODS: Conserved primers...
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