Article
New design and optimization of an in-house quantitative TaqMan Real-Time PCR-based assay for the detection and monitoring of occult hepatitis B virus (genotype A-J) infection.
Indian journal of medical microbiology - 1 Jan 2000
Ghorbani Mohammad, Shokri Rahman, Kia Vahid, Yari Fatemeh, Sharifi Zohreh, Paryan Mahdi
Abstract excerpt
PURPOSE: HBV DNA quantification is used for individuals with uninterpretable serological tests, occult HBV infections, decreasing the window period of the disease, and treatment follow-up. Although there are commercial qPCR assays, they are expensive. In this study, we developed a highly sensitive quantitative TaqMan Real-Time PCR with an exogenous internal control to quantify HBV DNA in serum/plasma. METHODS: A...
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